Welcome

 Marcelo Fernandez-Viña

Dear Colleagues,

Welcome to the official website of the 17th International HLA and Immunogenetics Workshop (IHIWS). The tradition of the International HLA and Immunogenetics Workshop is strong and compelling. Sixteen prior workshops have provided the opportunity and momentum for major technological and scientific progress.

The 17th Workshop promises to further advance the continued international collaboration in research in HLA and Immunogenetics projects. The major goals are to define HLA and KIR genomics, map serologic epitopes using NGS and single antigen bead technologies, and develop robust research and clinical informatics tools for HLA and KIR.

We hope you will join us at the 17th International HLA and Immunogenetics Workshop to be held at the Asilomar Conference Grounds in Pacific Grove, California, September 6 – 10, 2017. This will be followed by the American Society of Histocompatibility and Immunogenetics (ASHI) 44th Annual Meeting September 11 – 15, 2017 in San Francisco, California.

Please set aside these important dates in 2017. We hope to see you in the San Francisco Bay Area during this time. Learn how you can participate, and keep up with the latest developments by signing up for email updates.

Yours Sincerely,
Marcelo Fernandez-Viña, Chair


About the Workshop / How to Participate


Components & Projects

The 17th IHIWS will study HLA and KIR genomics, define full length MHC sequences, map serologic epitopes and develop robust informatics for HLA and KIR research through the use of NGS, single antigen beads (SAB) and flow cytometry.

The 17th IHIWS projects will fall under the following components:

(Click on the component name for more information)

All Serologic Epitopes Projects All NGS of Full-length HLA Genes Projects NGS of Full-length KIR Genes Mapping of Serologic Epitopes Hematopoietic Cell Transplantation (HCT) Quality Control & Quality Assurance Informatics of Genomic Data NGS of Full-length HLA genes

Latest Developments (Read All)

NGS of Full-length HLA genes NGS of Full-length HLA genes -

The HLA genes are the most polymorphic loci in the human genome; over 12,000 genetic variants (alleles) have been identified at 19 HLA genes, with some individual genes displaying several thousand alleles. The allelic and structural variation that characterizes these genes pose extreme challenges for routine genotyping of these genetic loci; this variation cannot be characterized by a few SNPs, and the high level of polymorphism confounds de novo sequence assembly efforts. Until recently, HLA genotyping has been accomplished using a variety of PCR-based approaches involving sequence-specific oligonucleotide probe (SSOP) hybridization and/or Sanger sequencing of 400-600 base pair amplicons. These... Read More

Component: NGS of full length HLA genes: Preliminary results of the Pilot Study -

Lisa E Creary1, Steven J Mack2 and Marcelo Fernandez-Vina1
1Department of Pathology, Stanford Blood Center
2Children’s Hospital Oakland Research Institute

Overview

The ultimate goal of the 17th International HLA and Immunogenetics Workshop (IHIW) is to advance the fields of Histocompatibility and Immunogenetics (H & I) research through the application of Next-Generation Sequencing (NGS) technologies for HLA and KIR genotyping, and to advance the development of NGS technologies tailored to meet the needs of the H & I community.

In 2014, we initiated an international multi-center pilot study in order to assess the performance of various NGS protocols, platforms,... Read More

T Cell Receptor (TCR)-HLA Interaction -

The T cell receptor is the natural receptor of the HLA-peptide complex and interacts directly with MHC molecules, notably its CDR regions.  The goal of this project will be to combine analysis of TCR and HLA in the context of disease, vaccination/immune alterations and populations.  We would expect samples to be CD4 or CD8 T cells and to have full HLA class I and class II typing by NGS.

Project Leaders:

Sami Djoulah, PhD. (sami.Djoulah@wiratech.com)
Emmanuel Mignot MD, PhD (mignot@stanford.edu)

Extension of HLA allele sequences by full-length HLA allele-specific hemizygous Sanger sequencing (SSBT) -

The Human Leucocyte Antigen (HLA) is one of the most polymorphic gene systems present in the human genome and due to this high polymorphism, the golden standard for typing at the allele level has been and still is sequence based typing. Since sequencing strategies have mainly focused on identification of the peptide binding groove, therefore the majority of HLA alleles lack full length sequence information.

The goal of this project is to extend the sequences of as many incompletely covered HLA alleles as possible by full length unambiguous Sanger Sequencing. Although NGS approaches are currently implemented in several laboratories, many... Read More