Analysis of DSA Epitopes and Graft Outcomes

This project requires serum & DNA or complete HLA typing information by NGS HLA from donor/recipient pairs, first transplant, single organ (heart or kidney) where DSA present. Graft outcome information is required. Results obtained from various epitope analysis will be compared to solid phase results and NGS HLA typing from donor and recipient. Solid phase testing to be performed using standardized work shop protocols, custom and commercial reagents including IgG SAB I, IgG SAB II, Bio-C1q SAB I, Bio-C1q SAB II.

Sub-Projects (based on cohort and analysis performed)

  • Graft outcomes in the presence of antibodies to target epitopes: Aim is to evaluate graft outcomes in the presence of antibodies to target epitopes identified by NGS HLA typing
  • Differential immunogenicity of HLA epitopes: Aim is to define the immunogenicity of the individual epitopes
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